Scientists

Kevin Gaido

Kevin Gaido

Education

B.S., biochemistry, University of Notre Dame, Notre Dame, Indiana, 1982.
Ph.D., pharmacology and toxicology, West Virginia University Medical Center, Morgantown, West Virginia, 1986.
Postdoctoral training, CIIT Centers for Health Research, 1986-1989.

Research

My research specialty is the field of receptor-mediated mechanisms of toxicity. Current interests focus on characterizing chemical interaction with steroid hormone receptors as well as characterizing the resulting cellular and molecular responses. My research incorporates the use of genomic technologies with in vitro and in vivo models of endocrine action.

Selected Publications

Kuhl, A. J., Ross, S. M., and Gaido, K. W. (2007). Adapting in vivo DNase I footprinting to measure semi-quantitative changes in protein-DNA interactions following phthalate exposure. Anal. Biochem. In Press.

Gaido, K. W., Hensley, J. B., Liu, D., Wallace, D. G., Borghoff, S., Johnson, K. J., Hall, S. J., and Boekelheide, K. (2007). Fetal mouse phthalate exposure shows that gonocyte multinucleation is not associated with decreased testicular testosterone. Toxicol. Sci. 97(2), 491-503.

Lahousse, S., Wallace, D., Liu, D., Gaido, K. W., and Johnson, K. (2006). Testicular gene expression profiling following pubertal rat mono-(2-ethylhexyl) phthalate exposure suggests a conserved initial genetic response at fetal and pubertal ages. Toxicol. Sci. 93, 369-381.

Mu, X., Liu, K., Kleymenova, E., Sar, M., Young, S. S., and Gaido, K. W. (2006). Gene expression profiling of androgen receptor antagonists in the rat fetal testis reveals few common gene targets. J. Biochem Mol. Toxicol. 20, 7-17.

Andersen, M. E., Thomas, R. S., Gaido, K. W., and Conolly, R. B. (2005). Dose-response modeling in reproductive toxicology in the systems biology era. Reprod. Toxicol. 19, 327-337.

Bowman, C. J., Turner, K. J., Sar, M., Barlow, N. J., Gaido, K. W., and Foster, P. M. D. (2005). Altered gene expression during rat Wolffian duct development following di(n-butyl) phthalate exposure. Toxicol. Sci. 86, 161-174.

Kleymenova, E., Swanson, C., Boekelheide, K., and Gaido, K. W. (2005). Exposure in utero to di(n-butyl)phthalate alters the vimentin cytoskeleton of fetal rat sertoli cells and disrupts sertoli cell-gonocyte contact. Biol. Reprod. 73, 482-490.

Liu, D., Gaido, K. W., and Wolfinger, R. (2005). Analysis of variation of amplitudes in cell cycle gene expression. Theor. Biol. Med. Model. 2, 46.

Liu, K., Lehmann, K. P., Sar, M., Young, S. S., and Gaido, K. W. (2005). Gene expression profiling following in utero exposure to phthalate esters reveals new gene targets in the etiology of testicular dysgenesis. Biol. Reprod. 73(1), 180-192.

Liu, K., Phadke, D., Gong, X., Cooper, D. C., Gaido, K. W., and Young, S. S. (2005). PowerArray: an integrated analysis environment for high dimensional data. Bioinformatics. In press.

National Toxicology Program (2005). NTP-CERHR Expert Panel Update on the Reproductive and Developmental Toxicity of Di(2-ethylhexyl) phthalate. NTP-CERHR-DEHP-05.

Thompson, C. J., Ross, S. M., Hensley, J., Liu, K., Heinze, S. C., and Gaido, K. W. (2005). Gene expression profiling reveals a rapid and dynamic response of the fetal testis to di(n-butyl) phthalate. Biol. Reprod. 73, 908-917.

Wyde, M. E., Kirwan, S. E., Zhang, F., Laughter, A., Hoffman, H. B., Bartolucci-Page, E., Gaido, K. W., Yan, B., and You, L. (2005). Di-n-butyl phthalate activates constitutive androstane receptor and pregnane X receptor and enhances the expression of steroid-metabolizing enzymes in the liver of rat fetuses. Toxicol. Sci. 86, 281-290.

Lehmann, K. P., Phillips, S. Sar, M., Foster, P. M. D., and Gaido, K. W. (2004). Dose-dependent alterations in gene expression and testosterone synthesis in the fetal testes of male rats exposed to di(n-butyl) phthalate. Toxicol. Sci. 81, 60-68.

Barlow, N. J., Phillips, S. L., Wallace, D. G., Sar, M., Gaido, K. W., and. Foster, P. M. D. (2003). Quantitative changes in gene expression in fetal rat testes following exposure to di(n-butyl) phthalate. Toxicol. Sci. 73, 431-441.

Gaido, K. W., You, L., and Safe, S. (2003). Modification of endocrine active potential by mixtures. Pure Appl. Chem. 75, 2069-2079.

Thompson, C. J., Ross, S. M., and Gaido, K. D. (2003). Di(n-butyl) phthalate impairs cholesterol transport and steroidogenesis in the fetal rat testis through a rapid and reversible mechanism. Endocrinology 145, 1227-1237.

Porter, K. L., Chandra, S., Wang, H. Q., Gaido, K. W., Smart, R. C., and Robinette, C. L. (2002). 17?-estradiol is a hormonal regulator of mirex tumor promotion sensitivity in mice. Toxicol. Sci. 69, 42-48.

Safe, S. H., Pallaroni, L., Yoon, K., Gaido, K., Ross, S., and McDonnell, D. (2002). Problems for risk assessment of endocrine-active estrogenic compounds. Environ. Health Perspect. 110, 925-929.

Tamura, H., Yoshikawa, H., Gaido, K. W., Ross, S. M., DeLisle, R. K., Welsh, W. J., and Richard, A. M. (2002). Interaction of organophosphate pesticides and related compounds with the androgen receptor. Environ. Health Perspect. 111, 545-552.

Turner, K. J., Barlow, N. J., Struve, M. F., Wallace, D. G., Gaido, K. W., Dorman, D. C., and Foster, P. M. D. (2002). Effects of in utero exposure to the organophosphate insecticide fenitrothion on androgen dependent reproductive development in the Crl:CD(SD)BR rat. Toxicol. Sci. 68, 174-183.

You, L., Casanova, M., Bartolucci, E. J., Fryczynski, M. W., Dorman, D. C., Everitt, J. I., Gaido, K. W., Ross, S. M., Heck, H. d'A. (2002). Combined effects of the dietary phytoestrogen and synthetic endocrine-active compound on reproductive development in Sprague-Dawley rats: genistein and methoxychlor. Toxicol. Sci. 66, 91-104.

Foster, P. M., Mylchreest, E., Gaido, K. W., and Sar, M. (2001). Effects of phthalate esters on the developing reproductive tract of male rats. Hum. Reprod. Update 7, 231-235.

Safe, S. H., Pallaroni, L., Yoon, K., Gaido, K., Ross, S., Saville, B., and McDonnell, D. (2001). Toxicology of environmental estrogens. Reprod. Fertil. Dev. 13, 307-315.

Shultz, V. D., Phillips, S., Sar, M., Foster, P. M., and Gaido, K. W. (2001). Altered gene profiles in fetal rat testes after in utero exposure to di(n-butyl) phthalate. Toxicol. Sci. 64, 233-242.

Waters, K. M., Safe, S., and Gaido, K. W. (2001). Differential gene expression in response to methoxychlor and estradiol through ERalpha, ERbeta, and AR in reproductive tissues of female mice. Toxicol. Sci. 63, 47-56.

Yoon, K., Pallaroni, L., Stoner, M., Gaido, K., and Safe, S. (2001). Differential activation of wild-type and variant forms of estrogen receptor ? by synthetic and natural estrogenic compounds using a promoter containing three estrogen-responsive elements. J. Steroid Biochem. Mol. Biol. 76, 25-32.

McIntyre, B. S., Barlow, N. J., Wallace, D. G., Maness, S. C., Gaido, K. W., and Foster, P. M. D. (2000). Effects of in utero exposure to linuron on androgen-dependent reproductive development in the male Crl:CD(SD)BR rat. Toxicol. Appl. Pharmacol. 167, 87-99.

Yoon, K., Pellaroni, L., Ramamoorthy, K., Gaido, K., and Safe, S. (2000). Ligand structure-dependent differences in activation of estrogen receptor alpha in human HepG2 liver and U2 osteogenic cancer cell lines. Mol. Cell. Endocrinol. 162, 211-220.